Premium
Antibodies against an intermediate on the water side of photosystem II of photosynthesis
Author(s) -
Zilinskas Barbara
Publication year - 1972
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(72)80472-1
Subject(s) - library science , citation , botany , computer science , biology
The chemical intermediates of the dark reactions of Photosystem II (PS II)* associated with the evolution of oxygen from water are unknown. Work with chemical inhibition [ 1, 21 , UV light inactivation [3], and heating effects (41 seem to indicate that at least one chemical component, probably proteinaceous in nature, is involved. the isolation and characterization of which have not been possible due to its extreme lability. Honeycutt and Krogman [5] have shown an additional site of inhibition by phenylmercuric acetate, located between the Tris and 3-(3,4-dichlorophenyl)-1, 1 dimethylurea (DCMU) blocks. We have found that antibodies made against PS II particles specifically inhibit oxygen evolution at a site between MnCl, donation [6] and diphenylcarbazide (DPC) donation [7] , that is probably against the same component inactivated (or extracted) by high concentrations of Tris buffer. The fact that this component is antigenic suggests that it is most likely either a protein of significant molecular weight or is complexed to a carrier molecule. The immunological technique has been well exploited as far as Photosystem I (PS I) intermediates are concerned. Antibodies have been directed against