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Peptidyl puromycin synthesis; effect of several antibiotics which act on 50 S ribosomal subunits
Author(s) -
Tanaka Kentaro,
Teraoka Hiroshi,
Tamaki Mikio
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80666-x
Subject(s) - puromycin , oleandomycin , spiramycin , tylosin , chemistry , ribosome , ribosomal rna , chloramphenicol , antibiotics , stereochemistry , biochemistry , protein biosynthesis , tetracycline , erythromycin , rna , gene
The effects of several antibiotics which are know to bind with 50 S ribosomal subunits, on the formation of several di‐ and tri‐peptidyl puromycins have been examined. Tylosin and spiramycin inhibited the formation of phenylalanyl‐ 14 C‐phenylalanyl‐puromycin, glycyl‐ 14 C‐phenyllalanyl‐puromycin, leucyl‐ 14 C‐phenylalanyl‐puromycin, N ϵ ‐carbobenzoxylysyl‐ 14 C‐phenylalanyl‐puromycin, and valyl‐glycyl‐ 14 C‐phenylalanyl‐puromycin as well as N ‐acetyl‐ 14 C‐phenylalanyl‐puromycin. Of these compounds, erythromycin and oleandomycin selectively inhibited the formation of phenylalanyl‐ 14 C‐phenylalanyl‐puromycin. Although chloramphenicol and lincomycin inhibited the formation of most of these peptidyl puromycins, the formation of phenylalanyl‐ 14 C‐phenylalanyl‐puromycin and leucyl‐ 14 C‐phenylalanyl‐puromycin was found to be resistant to these antibiotics. So far, no significant effect of siomycin has been observed on pepetidyl puromycin formation in the absence of G factor.