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Reconstitution of the 50 S ribosome subunit. Localization of activities related to the peptidyl transferase centre
Author(s) -
Ballesta Juan P.G.,
Montejo Vicente,
Vazquez David
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80609-9
Subject(s) - ribosome , protein subunit , microbiology and biotechnology , peptidyl transferase , biology , genetics , rna , gene
The peptidyl transferase centre, responsible for the catalytic activity required for the peptide bond formation step in protein synthesis, is an integral part of the larger ribosomal subunit in procaryotic [ 1 ] ns well as eucaryotic [2] cells. To investigate this Pctive centre we have developed adequate systeI;ls to study: (a) peptidyl transferase catalytic activiLy, (b) substrate interaction at the Pand A-sites of the centre and (c) interaction of a number of antibiotics. These assays are particularly useful to investigate the above activities in the reconstitution of 50 S ribosome subunits in order to know their requirements and localization. The results obtained are reported in this work.