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Der einfluss von coffein auf das schmelzverhalten von DNS
Author(s) -
Lang H.
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80398-8
Subject(s) - chemistry , premelting , caffeine , hyperchromicity , dna , denaturation (fissile materials) , biochemistry , biology , organic chemistry , nuclear chemistry , melting point , endocrinology
The interaction of caffeine with DNA has been studied at several ionic strengths by heat denaturation. At 2 mM K + caffeine produces a stabilization, but at 20 mM K + or higher a destabilization of secondary structure of DNA occurs. There is a correlation between heterogeneity and destabilization of DNA in presence of caffeine. In the premelting region a hyperchromic effect can be observed. It is suggested that two binding processes exists in the DNA‐caffeine interaction.