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Fraktionierung handelsüblichen insulins. Gewinnung und eigenschaften einer hochmolekularen, insulin‐ähnlichen komponente
Author(s) -
Zühlke H.,
Kohnert K.-D.,
Ziegler M.,
Knospe S.,
Behlke J.
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80300-9
Subject(s) - sephadex , size exclusion chromatography , trypsin , chemistry , ultracentrifuge , insulin , incubation , chromatography , biochemistry , biology , endocrinology , enzyme
A component with high molecular weight is prepared from commercial bovine insulin by gel filtration on Sephadex G 50. The substance has little biological and immunological activity in comparison to insulin. The activity is not changed by incubation with trypsin. It associates in 1 M CH 3 COOH and possesses a molecular weight of 28,500 as shown by ultracentrifugation. The ability of the component to precipitate with bovine insulin antibodies demonstrates that it contains immunologically active insulin‐like proteins and is not a homogenous substance.