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Optical rotatory dispersion and circular dichroism of baker's yeast transketolase in the far ultraviolet region of the spectrum
Author(s) -
Kochetov G.A.,
Usmanov R.A.,
Merzlov V.P.
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80263-6
Subject(s) - bioorganic chemistry , optical rotatory dispersion , chemistry , circular dichroism , physics , state (computer science) , stereochemistry , mathematics , organic chemistry , enzyme , algorithm
Analysis of smooth optical rotatory dispersion curves of apotransketola~ after Shechter and Blout [l] reveals marked differences in the degree of LYspiralization, determined by the Arss and Azzs [2] . Study of the pH dependence of optical rotation [2] has shown that these differences could be due both to the apolar environment of the o-helical segments and to the contribution of the &forms. Optical activity in the far UV region of the spectrum furnishes valuable information about the structure of the protein molecule. The present paper describes the data on optical rotatory dispersion and circular dichroism of transketolase in the absorption region of the peptide chromophore.