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The function of pseudouridylic acid in transfer ribonucleic acid. Irradiation and cyanoethylation of E. coli valine tRNA fragments
Author(s) -
Krauskopf Manuel,
Ofengand James
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80034-0
Subject(s) - citation , transfer rna , chemistry , valine , physics , library science , biochemistry , computer science , rna , amino acid , gene
In the previous paper [ I] , we have described some of the structural requirements for the lightinduced cross-linking reaction between the two non-adjacent bases, 4-Srds and Cydis, of tRNAfMet and the use of this reaction as a tool to probe the extent of structural changes induced by cyanoethylation of the \krd residue of this molecule. In this report, we extend these results to tRNA;Va’ of E. coli, a non-initiator tRNA species. It has already been shown by Yaniv and coworkers [2,3] that irradiation of tRNAVa’ at 335 nm induces a covalent cross-link between 4-Srds and Cydra and that endonuclease digestion of the tRNA prevents the reaction. Moreover, the molecule can be fragmented into two halves (fig. l), which when recombined and reannealed, regenerate acceptor activity [6]. The 5’half contains both of the residues involved in the cross-link and the 3’-half contains the only \krd residue. By the use of these half molecules of tRNA, we have been able to show that tRNAfMe* [l] , the 3’fragment is necessary for the 5’-fragment to become intramolecularly cross-linked. On the other hand, unlike tRNAfMe*, cyanoethylation of the 3’-fragment