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Effects of tannic acid and 2‐thenoyltrifluoroacetone on succinate oxidation and penetration across the mitochondrial membrane
Author(s) -
Luciani S.
Publication year - 1971
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(71)80023-6
Subject(s) - thenoyltrifluoroacetone , tannic acid , chemistry , penetration (warfare) , mitochondrion , biochemistry , nuclear chemistry , chromatography , organic chemistry , mathematics , operations research , extraction (chemistry) , solvent extraction
The penetration of succinate across the mitochondrial membrane is a rate limiting step in the oxidation of this substrate in isolated intact mitochondria [l] whereas in mitochondrial particles the oxidation of succinate is only dependent upon the activity of the succinate oxidase system. ‘lTA* is a known specific inhibitor of succinate CoQ-reductase activity [2, 31 and also of succinate oxidation both in intact mitochondria [4-61 and in submitochondrial particles [3]. Tannic acid, at concentrations which inhibit succinate oxidation in mitochondria, is devoid of any effect on submitochondrial particles [7] . TTA is a chelating agent for iron [8] and its effect on succinate oxidase has been generally ascribed to interaction with nonheme iron [2-61. Tannic acid though having the capacity to precipitate ferrous salts is essentially a protein cross-linking reagent [9] and its effect on succinate oxidation (only in intact mitochondria) together with its inhibition of mitochondrial swelling in isotonic ammonium succinate has been explained by suggesting an interaction of this drug with the hypothetic exchange diffusion carrier of succinate [lo] . However, in the course of the study on the mechanism of action of tannic acid, it has been observed that increasing the concentration of this drug caused an inhibition of succinate oxidation also in submitochondrial particles [ 1 l] . On the basis of this result and of the recent proposal by Tyler and Newton [ 121 that nonheme iron