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Formation of initiation complex with λmRNA in vitro
Author(s) -
Becarevic Aleksandar
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80556-7
Subject(s) - ribosome , messenger rna , in vitro , chemistry , initiation factor , protein biosynthesis , transcription (linguistics) , rna , amino acid , peptide , rna polymerase ii , microbiology and biotechnology , biochemistry , biology , gene expression , gene , promoter , linguistics , philosophy
The product from the in vitro transcription of native λDNA by purified E. coli RNA polymerase does not stimulate total amino acid incorporation although it can program ribosomes for the first peptide bond synthesis in the presence of the three initiation factor A, B and C.