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Studies on the half life time of rat liver transfer RNA species
Author(s) -
Hanoune Jacques,
Agarwal M.K.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80496-3
Subject(s) - transfer rna , rna , orotic acid , chemistry , chromatography , biochemistry , half life , biology , pharmacokinetics , pharmacology , gene
The tRNA fraction, extracted from very high speed supernatant fluid, from livers of rats injected with 3 H‐orotic acid, attained maximum specific activity after a little over 24 hr and, thereafter, decayed with an apparent half life of 5 days. This behaviour of tRNA was indistinguishable from that of liver rRNA and the acid soluble pool. Chromatography of tRNA, doubly labelled during a period of short synthesis and of prolonged decay, on a BD cellulose column, indicated that individual tRNA species turn over at a constant rate with respect to one another.