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Energy‐linked reactions in mitochondria: A requirement for ubiquinone after pentane extraction
Author(s) -
Sweetman A.J.,
Griffiths D.E.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80424-0
Subject(s) - library science , citation , chemistry , computer science
A role for ubiquinone in oxidative phosphorylation and related reactions has been suggested by many workers and many hypotheses have been advanced to explain the role of quinones in energy conservation reactions. There is little or no evidence which directly supports the hypotheses that have been proposed and the role of ubiquinone remains obscure [ 1,2] . We have investigated the effect of pentane extraction on energy-linked reactions in submitochondrial particles prepared from lyophilised beef heart mitochondria. The reactions studied were the energylinked transhydrogenase reaction (ATPdriven) and the energy-linked reduction of NADt by succinate (ATPdriven). Pentane extraction by the method of Szarkowska [3] as modified by Horio et al. [4], caused complete loss of energy-linked activities in derived submitochondrial particles. The energy-linked transhydrogenase reaction in submitochol:drial particles could be restored by addition of the pentane extract or ubiquinone alone to the lyophilised extracted mitochondria. This was a specific effect of ubiquinone and it could not be achieved by addition of vitamin K1 or by addition of phospholipids. The energy-linked reduction of NAIY by succinate could not be restored by ubiquinone alone but could be restored by the pentane extract