Premium
The inhibition of ribonucleic acid synthesis by the thiol‐oxidizing agent, diamide, in Escherichia coli
Author(s) -
Zehavi-Willner Tova,
Wax Richard,
Kosower Edward M.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80323-4
Subject(s) - oxidizing agent , glutathione , escherichia coli , thiol , cysteine , biochemistry , chemistry , protein biosynthesis , rna , uracil , strain (injury) , biology , organic chemistry , enzyme , dna , gene , anatomy
The thiol‐oxidizing agent, diamide, has been used to convert glutathione to glutathione disulfide within the cells of a stringent strain of Escherichia coli (CP 78), leading to a cessation of 14 C‐leucine incorporation (protein synthesis) and 3 H‐uracil incorporation (RNA synthesis). Parallel experiments with an isogenic relaxed strain (CP 79) gave similar results, providing evidence that glutathione is closely linked to RNA synthesis indepently of the link previously shown to protein synthesis.