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Partial amino acid sequence of the variable region of a mouse γG2a immunoglobulin heavy chain. Evidence for the existence of a third sub‐group of variability for the heavy chain pool
Author(s) -
Bourgois Alain,
Fougereau Michel
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80283-6
Subject(s) - citation , sequence (biology) , combinatorics , microbiology and biotechnology , chemistry , stereochemistry , philosophy , physics , mathematics , biology , library science , computer science , biochemistry
Subgroups of variability have been described for both the light [l-3] and the heavy chains [4-81 of human immunoglobulins. In a previous publication [9] , we have reported the isolation and characterization of CNBr fragments of murine plasmocytoma protein MOPC 173. Ten fragments, HI to HlO were assigned to the heavy chains, and provisionally ordered. Fragments Hl, H2, and H3 contained 34,48 and 21 amino acid residues, respectively, and therefore accounted for the major part of the variable region of the heavy chains [4, 6,8]. In the present communication, we report the sequence of fragments Hl and H3, linked together through a disulfide bond, and representing a total of 55 residues, thus covering approximately half of the Hv region.