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Polarized fluorescence of acridine orange‐transfer RNA complexes
Author(s) -
Surovaya A.,
Borisova O.,
Jilyaeva T.,
Scheinker V.,
Kisselev L.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80263-0
Subject(s) - chinese academy of sciences , library science , biological sciences , physics , biology , political science , computational biology , computer science , china , law
Previously we attempted to analyse the molecular morphology of mixed tRNAs from the rotational relaxation time (ph) measured from fluorescence polarization of acridine orange (AO) adsorbed on the tRNA molecules [ 1 ] . The same approach was also used by Churchich [2] and by Millar and Steiner [3] using a dye covalently attached to the acceptor end of the mixed tRNA molecules. The ph values they found were, however, considerably lower than when the dye was adsorbed on the double-helical regions of tRNA [l] . This difference is probably attributable to the local mobility of the dye coupled with the relatively flexible ACC end in Mg2+-free medium. The aim of this work was to carry out similar investigations on two individual tRNA fractions. Moreover, calculations were made taking into account various orientations of the dye molecule with respect to the long axis of the tRNA particle in solution and also various molecular volumes of the tRNA depending on the degree of its hydration.