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Purification of soluble murine transplantation antigens by isoelectric focusing
Author(s) -
Jolles P.,
Schoentgen F.,
Halle-Pannenko O.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80254-x
Subject(s) - isoelectric focusing , isoelectric point , antigen , hemagglutinin (influenza) , transplantation , chemistry , microbiology and biotechnology , antibody , acrylamide , gel electrophoresis , polyacrylamide gel electrophoresis , chromatography , biochemistry , microsome , biology , immunology , in vitro , medicine , enzyme , monomer , surgery , organic chemistry , polymer
Hydrosoluble transplantation antigens were prepared from membranes and microsomes of C 3 H mice (BP8 tumoral cells) and purified by isoelectric focusing. A biologically active fraction which seems homogenous by acrylamide gel electrophoresis was characterized: it specifically inhibits anti C 3 H hemagglutinin antibodies and provokes a highly significant prolongation of skin graft.