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An initiation factor causing dissociation of E. coli ribosomes
Author(s) -
Albrecht J.,
Stap F.,
Voorma H.O.,
Van Knippenberg P.H.,
Bosch L.
Publication year - 1970
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(70)80082-5
Subject(s) - thermolabile , ribosome , gtp' , dissociation (chemistry) , chemistry , biochemistry , protein biosynthesis , initiation factor , transfer rna , biophysics , messenger rna , stereochemistry , biology , rna , enzyme , organic chemistry , gene
Purification of crude initiation factors, essential for polypeptide synthesis in cell‐free systems of E. coli , yielded a fraction DF which causes dissociation of 70 S ribosomes. Its stoichiometric action on 70 S ribosomes is antagonized by increasing Mg 2+ concentrations but not by the addition of 30 S and 50 S subunits washed with high salt concentration. GTP did not stimulate this dissociating action. 2 μg of our most purified preparation caused 100% dissociation of 100 μg of 70 S ribosomes without added GTP. DF‐induced dissociation is a very rapid process at 37°C and is temperature‐dependent in the range of 0°–37°C. DF, which is thermolabile factor, is much less or not effective with complexed 70 S ribosomes bearing peptidyl‐tRNA and mRNA.