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The effect of mitochondrial oxidations of inhibitors of the dicarboxylate anion transporting system
Author(s) -
Robinson B.H.,
Williams G.R.
Publication year - 1969
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(69)80374-1
Subject(s) - chemistry , mitochondrion , ion , biochemistry , combinatorial chemistry , organic chemistry
It has been demonstrated that certain n-substituted malonic acids have an inhibitory effect on the oxidation of diand tri-carboxylate anions by rat liver mitochondria [l-3] . Thus the oxidations of citrate, isocitrate, cis-aconitate and 2-oxoglutarate which are de pendent on the additon of Lmalate as an activator of mitochondrial penetration [l] were shown to be inhibited by 2-n-butylmalonate, the inhibition being competitive with L-malate. The oxidations of L-malate with cysteine sulphinic acid and succinate, both dependent on the penetration of the mitochondria by a dicarboxylate anion, were also inhibited by 2-n-butylmalonate [2]. The kinetics of the inhibition caused by butylmalonate indicated that its site of action was at the level of the membrane transporting system for dicarboxylate anions. When this inhibitor was used on rat heart mitochondria it was found to inhibit oxidations not requiring the transport of dicarboxylate anions. These difficulties were partially overcome by the use of a new inhibitor, p-iodobenzylmalonate.