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Incorporation of fatty acids into the outer and inner membranes of isolated rat liver mitochondria
Author(s) -
Nachbaur J.,
Colbeau A.,
Vignais P.M.
Publication year - 1969
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(69)80113-4
Subject(s) - phosphatidylethanolamine , inner membrane , phospholipid , membrane , mitochondrion , biochemistry , inner mitochondrial membrane , phospholipase , bacterial outer membrane , phospholipase a2 , chemistry , oleic acid , enzyme , phospholipase a , phospholipase a1 , translocase of the outer membrane , phosphatidylcholine , atp–adp translocase , escherichia coli , gene
Acyl‐CoA: phospholipid acyl‐transferase activity as well as phospholipase A activity were detected in inner and outer membrane preparations from rat liver mitochondria. Both enzyme systems have an optimum pH around 8 and act preferentially on phosphatidylethanolamine. While phospholipase A activity is much lower in the inner membrane than in the outer membrane of mitochondria the reverse is true for the incorporation of ( 14 C)‐oleic acid into endogenous phosphatidylethanolamine. These results bring an indirect evidence that the inner membrane per se possesses a phospholipase A activity.