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pH induced changes in optical activity of guanine nucleosides
Author(s) -
Guschlbauer Wilhelm,
Courtois Yves
Publication year - 1968
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(68)80055-9
Subject(s) - guanosine , optical rotatory dispersion , guanine , protonation , chemistry , circular dichroism , conformational change , dichroic glass , cotton effect , biophysics , stereochemistry , crystallography , biochemistry , nucleotide , biology , physics , organic chemistry , optics , ion , gene
Optical rotatory dispersion and circular dichroism have been used to investigate the protonation of guanosine and some of its analogues. An inversion of the principal Cotton effect and the dichroic band is observed below the acid pK. It is suggested that a conformational change from the anti form above the pK to the syn form below the pK occurs. The reasons why this change should occur only in guanosine and not in adenosine are discussed.