Open AccessRapid removal of detergent in glycolipids using ionic liquids
Author(s) -
Shoko Matsushita,
Kohei Noda,
Shuta Hara,
Jumpei Aisu,
Aki Hayashi,
Hiroki Ikake,
Shigeru Shimizu,
Minoru Tanigawa,
Motohiko Ukiya,
Kayoko Nishimura,
Atsuyoshi Nishina,
Yusuke Suzuki
Publication year - 2022
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.1007/s44211-022-00139-x
Subject(s) - chemistry , glycolipid , chromatography , ionic liquid , membrane , sample preparation , iodide , organic chemistry , biochemistry , catalysis
Detergent removal in glycolipid after sample preparation, such as enzymatic reaction or isolation of detergent-resistant membrane microdomain, is indispensable for further structural characterization. We previously established the rapid and effective method of detergent removal in glycolipid samples from glass test tube using 1,2-dichloroethane (DCE) washing. However, the use of DCE has several drawbacks, such as environmental risks, harmful effects (potentially carcinogenic), and high vaporability and flammability. To solve the issue, we used ionic liquids to remove detergents from glycolipid samples, and found 1-butyl-3-methylimidazolium iodide was a suitable alternative for DCE.