Open AccessUtilizing dual carriers assisted by enzyme digestion chemiluminescence signal enhancement strategy simultaneously detect tumor markers CEA and AFP
Author(s) -
Xiaojun Yan,
Kun Zhao,
Yunting Yang,
Aojun Qiu,
Xinlei Zhang,
Jie Liu,
Zha Chengyi,
Xi Mai,
Fanrong Ai,
Xiangjuan Zheng
Publication year - 2022
Publication title -
analytical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 73
eISSN - 1348-2246
pISSN - 0910-6340
DOI - 10.1007/s44211-022-00109-3
Subject(s) - chemistry , horseradish peroxidase , carcinoembryonic antigen , aptamer , chemiluminescence , luminol , detection limit , linear range , hydroxylamine , chromatography , enzyme , microbiology and biotechnology , biochemistry , cancer , medicine , biology
To measure two tumor biomarkers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), a dual-carrier CL sensor with restriction enzyme digestion (Exo I) and aptamer technology utilizing gold nanoparticles (hydroxylamine amplification) and horseradish peroxidase (HRP) as the CL signal enhancement in the sensing strategy was formed. These nanoparticles and nano-enzyme were precisely detected and tagged to the appropriate position attributable to the particular recognition of biotin and streptavidin. In this sensing strategy, target markers were further enriched and recognized sensitively by CL following enrichment, and matching strong chemical signals were collected under luminol catalysis, allowing for marker identification. For CEA (0.1-80 ng/mL) and AFP (2-500 ng/mL), the proposed method has a large linear range, with detection limits of 36.6 pg/mL and 0.94 ng/mL, respectively.