Open AccessFlavonoid fractions of diosmin and hesperidin mitigate lead acetate-induced biochemical, oxidative stress, and histopathological alterations in Wistar rats
Author(s) -
Ibrahim Yusuf Lamidi,
Hudu Garba Mikail,
Ali Sani,
Isaac Oluwatobi Akefe,
Tareq Abed Mohammed,
Sabo Isa Salihu,
Aisha Omobolanle Olatunji,
Abdussalam Hassan,
N. Daniel,
Victoria Aderonke Adegoke
Publication year - 2021
Publication title -
toxicological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.872
H-Index - 30
eISSN - 2234-2753
pISSN - 1976-8257
DOI - 10.1007/s43188-020-00084-9
Subject(s) - diosmin , hesperidin , histopathology , lead acetate , malondialdehyde , antioxidant , oxidative stress , zinc protoporphyrin , flavonoid , pharmacology , chemistry , medicine , toxicity , biochemistry , enzyme , pathology , heme , alternative medicine
This study aims at investigating the protective effects of flavonoid fractions of diosmin and hesperidin in mitigating sub-chronic lead acetate-induced biochemical, oxidative stress, and histopathological alterations in adult male Wistar rats. Forty animals were randomly assigned into five groups, each consisting of eight animals. Group I animals was treated with deionised water only, group II, IV, and V were administered lead acetate 90 mg/Kg body weight (1/20th of the LD 50 ), groups III, and IV was administered Daflon (100 mg/Kg), while group V was administered Daflon (200 mg/Kg), 30 min prior treatment with lead acetate. All treatments lasted for 42 days. Blood lead levels, electrolyte parameters, zinc protoporphyrin (ZPP) levels, activities of antioxidant enzymes, and histopathology of vital organs, were evaluated following standard practice. Sub-chronic lead acetate exposure induced a decrease in levels of serum electrolytes, and activities of antioxidant enzymes, while blood lead levels, ZPP, and malondialdehyde levels were increased. Lead exposure also instigated marked variation in histopathology of vital organs. Conversely, co-treatment with graded doses of daflon improved the levels of blood lead, electrolytes, ZPP, activities of antioxidant enzymes, and histopathology of vital organs. Data obtained from the current study indicate that rats exposed to sub-chronic doses of lead acetate show increased blood lead levels, electrolyte imbalance, alongside impairment in ZPP levels, activities of antioxidant enzymes, and histopathology, while pretreatment using daflon mitigated the ensued perturbations. This, therefore, suggests that consumption of foods enriched with flavonoid fractions of diosmin and hesperidin may be beneficial for individuals inhabiting lead-polluted environments.