Open AccessIdentification of potential new mosquito-associated viruses of adult Aedes aegypti mosquitoes from Tocantins state, Brazil
Author(s) -
Matheus Almeida Duarte,
Fabrício Souza Campos,
Osvaldo F Araújo Neto,
Leonardo A. Silva,
Arthur Batista Silva,
Thalita C Aguiar,
Raíssa Nunes dos Santos,
Ueric José Borges de Souza,
Giselly Batista Alves,
Fernando Lucas Melo,
Daniel Mendes Pereira Ardisson-Araújo,
Raimundo Wagner Souza Aguiar,
Bergmann Morais Ribeiro
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00632-x
Subject(s) - aedes aegypti , biology , chikungunya , virology , dengue virus , aedes albopictus , arbovirus , dengue fever , virus , aedes , larva , ecology
Medically important arboviruses such as dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) are primarily transmitted by the globally distributed mosquito Aedes aegypti. Increasing evidence suggests that the transmission of some viruses can be influenced by mosquito-specific and mosquito-borne viruses. Advancements in high-throughput sequencing (HTS) and bioinformatics have expanded our knowledge on the richness of viruses harbored by mosquitoes. HTS was used to characterize the presence of virus sequences in wild-caught adult Ae. aegypti from Tocantins (TO) state, Brazil. Samples of mosquitoes were collected in four cities of Tocantins state and submitted to RNA isolation, followed by sequencing at an Illumina HiSeq platform. Our results showed initially by Krona the presence of 3% of the sequenced reads belonging to the viral database. After further analysis, the virus sequences were found to have homology to two viral families found in insects Phenuiviridae and Metaviridae. Three possible viral strains including putative new viruses were detected and named Phasi Charoen-like phasivirus isolate To-1 (PCLV To-1), Aedes aegypti To virus 1 (AAToV1), and Aedes aegypti To virus 2 (AAToV2). The results presented in this work contribute to the growing knowledge about the diversity of viruses in mosquitoes and might be useful for future studies on the interaction between insect-specific viruses and arboviruses.