Open AccessEvaluation of three different genomic regions for detection of bovine leukemia virus by real-time PCR
Author(s) -
Antônio Augusto Fonseca Júnior,
Luciana Rabello Ferreira,
Mateus Laguardia Nascimento,
Anselmo Vasconcelos Rivetti Júnior,
Bruna Lopes Bueno,
Jenner Karlisson Pimenta dos Reis,
Marcelo Fernandes Camargos
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00613-0
Subject(s) - bovine leukemia virus , repeatability , virology , real time polymerase chain reaction , biology , leukemia , virus , gene , genetics , chromatography , chemistry
Bovine leukemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus. BLV infects cattle worldwide and is responsible for significant economic losses. The objective of this study was to validate real-time quantitative PCR (qPCR) for the detection of BLV. After identification of the most efficient qPCR, the limits of detection, repeatability, and reproducibility were determined. The results indicate that qPCR can be easily reproduced between laboratories with high sensitivity. The test variation was low in samples from lesions suggestive of bovine leukosis or whole blood.