Open AccessExpression of F-actin and β-tubulin genes in free mycelia and robust biofilms of the filamentous fungus Aspergillus niger
Author(s) -
Luís Henrique Souza Guimarães,
Nilce Maria Martinez Rossi,
Tamires Aparecida Bitencourt
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00611-2
Subject(s) - mycelium , aspergillus niger , hypha , biology , biofilm , fermentation , microtubule , tubulin , context (archaeology) , microbiology and biotechnology , actin , cytoskeleton , fungal protein , actin cytoskeleton , biochemistry , saccharomyces cerevisiae , gene , botany , bacteria , cell , genetics , paleontology
The morphology and growth of the filamentous fungi are influenced by different factors as the culture conditions and the type of fermentative process. The production and secretion of metabolites by these organisms present a direct relationship with their morphology. The organization of the microtubules and actin in the cytoskeleton is determinant for both the fungal growth and morphology. In this context, this study aimed to analyze the expression of the β-tubulin, F-actin, and glucan synthase in the A. niger mycelia obtained from submerged fermentation and biofilm fermentation through qPCR, as well as the analysis of the nucleus distribution in the hypha. Herein, we showed that β-tubulin and the F-actin gene were more expressed in the biofilm condition, while the glucan synthase was in the submerged condition. No significant difference was observed in the nucleus distribution between the mycelia obtained from both the fermentative processes. In conclusion, the different morphologies observed for the mycelia from submerged fermentation and biofilm fermentation might be influenced by the differential modulation of genes that codify cytoskeleton proteins, which seems to be potentially regulated by mechanosensing during fungal contact with solid supports.