
Phenotypic and molecular identification of Brucella suis biotype 1 in a pig from Brazil—case report
Author(s) -
Acácia Ferreira Vicente,
Mateus de Souza Ribeiro Mioni,
Didier Quevedo Cagnini,
Márcio Garcia Ribeiro,
Marcelo Fagali Árabe Filho,
Fernando José Paganini Listoni,
Bruna Letícia Devidé Ribeiro,
Jane Megid
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00607-y
Subject(s) - brucella suis , brucellosis , serology , wild boar , orchitis , brucella , biology , zoonotic disease , veterinary medicine , virology , abortion , microbiology and biotechnology , disease , medicine , brucella melitensis , immunology , antibody , pathology , pregnancy , genetics
Brucellosis is a zoonotic disease with a global impact. Brucella suis is one of the most pathogenic species to humans, requiring different measures for the control and/or eradication of the disease. The serological investigation for brucellosis was performed in pigs, horses, dogs, and cattle on a farm with a history of abortion in sows and necropsy of a boar with severe necrosuppurative orchitis. One sow, two cows, and two dogs reveled positive to Rose Bengal Test (RBT), although only the sow had a confirmatory outcome in 2-mercaptoethanol (2-ME). The 2-ME-positive sow was euthanized and microbiological culture of lymph nodes and liver followed by biochemical characterization allowed phenotypic characterization of Brucella suis biotype 1. PCR multiplex Bruce-ladder and Suis-ladder enabled molecular confirmation, respectively, of Brucella suis and biotype 1. The transmission aspects of B. suis to pigs and other domestic species, the combination of diagnostic procedures to diagnosis, as well as human health concerns of brucellosis are discussed.