Open AccessHelicobacter pylori outer membrane vesicles induce expression and secretion of oncostatin M in AGS gastric cancer cells
Author(s) -
Malak Zoaiter,
Roudaisser,
Rouba HageSleiman,
Fadi Abdel-Sater,
Bassam Badran,
Zaher Zeaiter
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00490-7
Subject(s) - oncostatin m , helicobacter pylori , bacterial outer membrane , secretion , cancer , biology , helicobacter , gastritis , population , cytokine , cancer research , immunology , medicine , interleukin 6 , endocrinology , gene , biochemistry , genetics , environmental health , escherichia coli
Helicobacter pylori, a human pathogen that colonizes the stomach of 50% of the world's population, is associated with gastritis, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. Diseases are characterized by severe inflammatory responses in the stomach that are induced by various chemokines and cytokines. Recently, oncostatin M (OSM), an IL-6 family cytokine, was detected in early gastric cancer biopsies. In this study, we showed that Helicobacter pylori induced secretion of OSM and overexpression of its type II receptor OSMRβ (OSM/OSMRβ) in a human gastric adenocarcinoma cell line (AGS) over 24 h of infection. Furthermore, we showed that the induction of OSM and OSMRβ was carried out by heat-sensitive Helicobacter pylori outer membrane vesicle (OMV) protein. Collectively, our results established, for the first time, a direct relation between Helicobacter pylori OMVs and the OSM/OSMRβ signaling axis.