Open AccessSerologic evidence of West Nile virus and Saint Louis encephalitis virus in horses from Southern Brazil
Author(s) -
Matheus Nunes Weber,
Ana Cristina Sbaraini Mósena,
Letícia F. Baumbach,
Mariana S da Silva,
Raíssa Canova,
Débora Regina Lopes dos Santos,
Renata da Fontoura Budaszewski,
Livia Oliveira,
Michel M. Soane,
Natália B. Saraiva,
Fernanda Teixeira da Silva Bellucco,
Bruno Amaral Mazurek,
Gustavo Nogueira Diehl,
Laura H. V. G. Gil,
Mauro Riegert Borba,
Luís Gustavo Corbellini,
Cláudio Wageck Canal
Publication year - 2021
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-021-00474-7
Subject(s) - virology , encephalitis , serology , virus , biology , japanese encephalitis , west nile virus , antibody , immunology
Flaviviruses as West Nile virus (WNV), Saint Louis encephalitis virus (SLEV), Ilhéus virus (ILHV), and Rocio virus (ROCV) are previously reported in different Brazilian regions, but studies in Southern Brazil are still scarce. To improve the information regarding flaviviruses in Southern Brazil, horse serum samples were analyzed using RT-qPCR and a commercial ELISA-Ab against WNV followed by PRNT 75 . All 1000 samples analyzed by real-time RT-PCR resulted negative. The 465 subsampled samples were analyzed by a commercial ELISA-Ab against WNV, and the 18.5% (86/465) positive samples were further analyzed by PRNT 75 . In the PRNT 75 , 13/86 and 2/86 horses were positive for SLEV and WNV, respectively. It was observed that 5.8% (13/226) of the farms presented at least one positive animal for SLEV in PRNT 75 , whereas 0.9% (2/226) for WNV. Apart from the lower seroprevalences identified when compared to data previously reported in other Brazilian regions, our results suggest that public health professionals must be aware of the presence of these potential zoonotic pathogens.