Open AccessGenome constellations of rotavirus a isolated from avian species in Brazil, 2008–2015
Author(s) -
Laila Andreia Rodrigues Beserra,
Célia Marisa Rizzatti Barbosa,
Mikael Berg,
Paulo Eduardo Brandão,
Rodrigo Martins Soares,
Fábio Gregori
Publication year - 2020
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-020-00259-4
Subject(s) - rotavirus , biology , sanger sequencing , virology , phylogenetic tree , genotype , genetic diversity , genome , genetics , phylogenetics , gene , viral evolution , transmission (telecommunications) , virus , evolutionary biology , dna sequencing , population , medicine , environmental health , electrical engineering , engineering
Rotaviruses are members of the family Reoviridae and are a common cause of acute diarrhea in many mammalian and avian species. They are non-enveloped icosahedral particles and their genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1-4, VP6-7) and six nonstructural proteins (NSP1-6). Genotypes are defined based upon the diversity found in these genes and viral characterization plays a central role on epidemiological studies and prevention. Here we investigate the distribution of Brazilian RVAs genotypes in 8 chicken samples collected between 2008 and 2015 from different regions by RT-PCR, partial (Sanger) nucleotide sequencing and phylogenetic analysis from all rotavirus genes. Although the identified genotypes were typical from avian host species, when analyzed together, they form novel genetic constellations: G19-P[31]-I11-R6-C6-M7-A16-N6-T8-E10-H8 and G19-P[31]-I4-R4-C4-M4-A16-N4-T4-E4-H4. This study highlights that avian rotaviruses are widespread among commercial farms in Brazil, and the co-circulation of at least two different genomic constellations indicates that may present a way bigger genetic variability, that can be increased by the possible transmission events from other birds, lack of specific preventive measures, as well as the different viral evolution mechanisms.