Open AccessEvaluation of the susceptibility test of polymyxin B using the commercial test Policimbac®
Author(s) -
Tanise Vendruscolo Dalmolin,
Maiara dos Santos Carneiro,
Luíza Peres de Castro,
Fabiana Caroline Zempulski Volpato,
Priscila Lamb Wink,
Daiana de Lima-Morales,
Afonso Luís Barth
Publication year - 2020
Publication title -
brazilian journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 1678-4405
pISSN - 1517-8382
DOI - 10.1007/s42770-020-00246-9
Subject(s) - polymyxin , polymyxin b , broth microdilution , minimum inhibitory concentration , microbiology and biotechnology , colistin , medicine , mathematics , biology , antibiotics
Broth microdilution (BMD), the reference method to determine bacterial susceptibility to polymyxins, is a laborious and time-consuming technique. Policimbac® is a commercial test panel which uses lyophilized polymyxin B to determine the minimum inhibitory concentration for Gram-negative isolates. This study evaluated the performance of Policimbac® in comparison with BMD for 110 isolates. Although the Policimbac® presented a very low essential agreement, the categorical agreement with BMD was optimal. Policimbac® is an alternative approach to BMD for evaluating the susceptibility to polymyxin B.