Open AccessDifferentiation of Adipose Tissue–Derived CD34+/CD31− Cells into Endothelial Cells In Vitro
Author(s) -
Anoosha Forghani,
Srinivas V. Koduru,
Cong Chen,
Ashley N. Leberfinger,
Dino J. Ravnic,
Daniel J. Hayes
Publication year - 2019
Publication title -
regenerative engineering and translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 11
eISSN - 2364-4133
pISSN - 2364-4141
DOI - 10.1007/s40883-019-00093-7
Subject(s) - matrigel , cd31 , microbiology and biotechnology , endothelial stem cell , adipose tissue , vasculogenesis , cd34 , angiogenesis , progenitor cell , vascular endothelial growth factor , biology , chemistry , stem cell , in vitro , cancer research , endocrinology , biochemistry , vegf receptors
In this study, CD34 + /CD31 - progenitor cells were isolated from the stromal vascular fraction (SVF) of adipose tissue using magnetic activated cell sorting. The endothelial differentiation capability of these cells in vitro was evaluated by culturing them in vascular endothelial growth factor (VEGF) induced medium for 14 days. Viability, proliferation, differentiation and tube formation of these cells were evaluated. Cell viability study revealed that both undifferentiated and endothelial differentiated cells remained healthy for 14 days. However, the proliferation rate was higher in undifferentiated cells compared to endothelial differentiated ones. Upregulation of endothelial characteristic genes (Von Willebrand Factor (vWF) and VE Cadherin) was observed in 2D culture. However, PECAM (CD31) was only found to be upregulated after the cells had formed tube-like structures in 3D Matrigel culture. These results indicate that adipose derived CD34 + /CD31 - cells when cultured in VEGF induced medium, are capable differentiation into endothelial-like lineages. Tube formation of the cells started 3h after seeding the cells on Matrigel and formed more stable and connected network 24 h post seeding in presence of VEGF.