Open AccessCharacterization of the biosurfactant produced by Pesudomonas areuginosa strain R4 and its application for remediation pyrene-contaminated soils
Author(s) -
Mehdi Ahmadi,
Forud Niazi,
Shokouh Ghafari,
Sahand Jorfi
Publication year - 2021
Publication title -
journal of environmental health science and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.447
H-Index - 45
ISSN - 2052-336X
DOI - 10.1007/s40201-021-00617-w
Subject(s) - rhamnolipid , pyrene , critical micelle concentration , chemistry , environmental remediation , bioremediation , environmental chemistry , soil contamination , contamination , biodegradation , strain (injury) , hydrocarbon , salinity , nitrogen , bacteria , micelle , organic chemistry , pseudomonas aeruginosa , aqueous solution , biology , ecology , genetics , anatomy
A biosurfactant producing strain was isolated and the rhamnolipid type biosurfactant was extracted for soil washing of a synthetically and naturally hydrocarbon-contaminated soil. Following the primary screening, Pseudomonas aeruginosa strain R 4 was selected and the effect of the carbon and nitrogen source and the salinity on biosurfactant production was studied. Of the best results were observed for glucose as a carbon source, NH 4 Cl as a nitrogen source and salinity of 1.4%. The produced biosurfactant was a glycolipid type biosurfactant and reduced the surface tension to 32.5 mN/m with a critical micelle concentration (CMC) of 50 mg/L and production yield of 90 mg/L. Using produced biosurfactant, a pyrene desorption rate of 82% was observed in selected conditions for initial pyrene concentration of 200 mg/L.