Open AccessThe effect of Faecalibacterium prausnitzii and its extracellular vesicles on the permeability of intestinal epithelial cells and expression of PPARs and ANGPTL4 in the Caco-2 cell culture model
Author(s) -
Seyedeh Marzieh Moosavi,
Abbas Akhavan Sepahi,
Seyed Fazlollah Mousavi,
Farzam Vaziri,
Seyed Davar Siadat
Publication year - 2020
Publication title -
journal of diabetes and metabolic disorders
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.619
H-Index - 33
ISSN - 2251-6581
DOI - 10.1007/s40200-020-00605-1
Subject(s) - faecalibacterium prausnitzii , caco 2 , extracellular vesicles , vesicle , cell culture , intestinal permeability , microbiology and biotechnology , extracellular , intracellular , permeability (electromagnetism) , chemistry , cell , biology , biochemistry , membrane , immunology , feces , genetics
Gut microbiota such as Faecalibacterium prausnitzii play a major role in the regulation of gut barrier, inflammation and metabolic functions. Microbiota-derived extracellular vehicles (EVs) have been recently introduced as functional units mediating the eukaryotic and prokaryotic cell-microbiota interactions. In this paper, the effect of F. prausnitzii and its EVs on mRNA expression levels of tight junction genes ( ZO1 and OCLN ) as well as PPARs and ANGPTL4 genes in the human epithelial colorectal adenocarcinoma (Caco-2) cell line was evaluated.