Open AccessSynergistic Effect of Laminin and Epidermal Growth Factor on Biological and Morphological Properties of Co-Cultured Myoblasts and Fibroblasts
Author(s) -
Mohd Asyraf Mat Afandi,
Manira Maarof,
Shiplu Roy Chowdhury,
Ruszymah Bt Hj Idrus
Publication year - 2020
Publication title -
jo'jig gonghag gwa jaesaeng uihag/tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.719
H-Index - 23
eISSN - 2212-5469
pISSN - 1738-2696
DOI - 10.1007/s13770-020-00283-3
Subject(s) - laminin , epidermal growth factor , myocyte , cell growth , rho kinase inhibitor , chemistry , cell migration , population , cell , microbiology and biotechnology , biology , endocrinology , kinase , receptor , rho associated protein kinase , medicine , biochemistry , environmental health
One of the long-standing problems of myoblasts in vitro expansion is slow cell migration and this causes fibroblast population to exceed myoblasts. In this study, we investigated the synergistic effect of laminin and epidermal growth factor (EGF) on co-cultured myoblasts and fibroblasts for cell attachment, proliferation and migration.