Open AccessGeneration of Scalable Hepatic Micro-Tissues as a Platform for Toxicological Studies
Author(s) -
Sara Darakhshan,
Ali Bidmeshki Pour,
Reza KowsariEsfahan,
Massoud Vosough,
Leila Montazeri,
Mohammad Hossein Ghanian,
Hossein Baharvand,
Abbas Piryaei
Publication year - 2020
Publication title -
jo'jig gonghag gwa jaesaeng uihag/tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.719
H-Index - 23
eISSN - 2212-5469
pISSN - 1738-2696
DOI - 10.1007/s13770-020-00272-6
Subject(s) - extracellular matrix , hepatic stellate cell , biology , cell culture , in vitro , microbiology and biotechnology , mesenchymal stem cell , matrigel , biochemistry , endocrinology , genetics
Currently, there is an urgent need for scalable and reliable in vitro models to assess the effects of therapeutic entities on the human liver. Hepatoma cell lines, including Huh-7, show weakly resemblance to human hepatocytes, limiting their significance in toxicity studies. Co-culture of hepatic cells with non-parenchymal cells, and the presence of extracellular matrix have been shown to influence the biological behavior of hepatocytes. The aim of this study was to generate the scalable and functional hepatic micro-tissues (HMTs).