Open AccessThe effects of acute doxorubicin treatment on proteome lysine acetylation status and apical caspases in skeletal muscle of fasted animals
Author(s) -
Dirks-Naylor Amie J.,
Tran Ngan T. K.,
Yang Sendra,
Mabolo Raean,
Kouzi Samir A.
Publication year - 2013
Publication title -
journal of cachexia, sarcopenia and muscle
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.803
H-Index - 66
eISSN - 2190-6009
pISSN - 2190-5991
DOI - 10.1007/s13539-013-0104-z
Subject(s) - sirtuin , doxorubicin , apoptosis , lysine , skeletal muscle , sirtuin 1 , acetylation , sod2 , pharmacology , caspase , medicine , cancer research , endocrinology , chemistry , biochemistry , oxidative stress , downregulation and upregulation , chemotherapy , programmed cell death , amino acid , superoxide dismutase , gene
Background Doxorubicin treatment is known to cause muscular weakness. However, the cellular mechanisms have not been elucidated. We aimed to determine the effects of acute doxorubicin treatment on proteome lysine acetylation status, an indication of the apoptotic and inflammatory environment, and the expression and activation of various apical caspases involved in the initiation of apoptosis. Methods Six‐week‐old male F344 rats were injected intraperitoneally with 20 mg/kg of doxorubicin or saline. Once the treatment was administered, both groups of animals were fasted with no food or water until sacrifice 24 h posttreatment. Results Doxorubicin treatment affected neither the proteome lysine acetylation status nor the expression of sirtuin 1, sirtuin 3, SOD1, or SOD2 in soleus of fasted animals. Doxorubicin treatment also did not affect the expression or activation of procaspase‐1, procaspase‐8, procaspase‐9, or procaspase−12. Conclusion We suggest that doxorubicin does not exert a direct effect on these catabolic parameters in skeletal muscle in vivo.