Open AccessEffect of colon cancer and surgical resection on skeletal muscle mitochondrial enzyme activity in colon cancer patients: a pilot study
Author(s) -
Phillips Bethan E.,
Smith Kenneth,
Liptrot Sarah,
Atherton Philip J.,
Varadhan Krishna,
Rennie Michael J.,
Larvin Mike,
Lund Jonathan N.,
Williams John P.
Publication year - 2013
Publication title -
journal of cachexia, sarcopenia and muscle
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.803
H-Index - 66
eISSN - 2190-6009
pISSN - 2190-5991
DOI - 10.1007/s13539-012-0073-7
Subject(s) - colorectal cancer , medicine , skeletal muscle , cancer , resection , oncology , gastroenterology , cancer research , surgery
Background Colon cancer (CC) patients commonly suffer declines in muscle mass and aerobic function. We hypothesised that CC would be associated with reduced muscle mass and mitochondrial enzyme activity and that curative resection would exacerbate these changes. Methods We followed age‐matched healthy controls and CC patients without distant metastasis on radiological imaging before and 6 weeks after hemi‐colectomy surgery. Body composition was analysed using dual energy X‐ray absorptiometry. Mitochondrial enzyme activity and protein concentrations were analysed in vastus lateralis muscle biopsies. Results In pre‐surgery, there were no differences in lean mass between CC patients and age‐matched controls (46.1 + 32.5 vs. 46.1 + 37.3 kg). Post‐resection lean mass was reduced in CC patients (43.8 + 30.3 kg, P < 0.01). When comparing markers of mitochondrial function, the following were observed: pyruvate dehydrogenase (PDH) activity was lower in CC patients pre‐surgery ( P < 0.001) but normalized post‐resection and cytochrome c oxidase and pyruvate dehydrogenase E2 subunit protein expression were lower in CC patients pre‐surgery and not restored to control values post‐resection ( P < 0.001). Nuclear factor kappa‐B, an inflammatory marker, was higher in CC patients pre‐surgery compared to controls ( P < 0.01), returning to control levels post‐resection. Conclusion Muscle mass was affected by surgery rather than cancer per se. PDH activity was however lower in cancer patients, suggesting that muscle mass and mitochondrial enzyme activity are not inextricably linked. This reduction in mitochondrial enzyme activity may well contribute to the significant risks of major surgery to which CC patients are exposed.