Open AccessTranscriptome sequencing analysis of porcine alveolar macrophages infected with PRRSV strains to elucidate virus pathogenicity and immune evasion strategies
Author(s) -
Fengxue Wang,
Xing Liu,
Hua Wu,
Yiping Wen
Publication year - 2021
Publication title -
virusdisease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.375
H-Index - 22
eISSN - 2347-3517
pISSN - 2347-3584
DOI - 10.1007/s13337-021-00724-0
Subject(s) - porcine reproductive and respiratory syndrome virus , biology , gene , transcriptome , virology , kegg , virus , immune system , innate immune system , virulence , gene expression , genetics
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes a serious disease to the swine industry worldwide. To understand the mechanisms of HP-PRRSV infection, RNA-seq-based transcriptome analyses were performed on porcine alveolar macrophages (PAMs) infected with a HP-PRRSV strain (TJ), a less virulent strain of a classical lineage (CH-1a), and a vaccine strain TJM-F92. Gene ontology, Kyoto Encyclopedia of Genes and Genomes analyses indicate that TJM-F92 led to significant up-regulation of gene expression for proteins associated with membrane-bound organelles. The differentially expressed genes of HP-PRRSV TJ-infected PAM cells were up-regulated in the special G-protein coupled receptor. The six cytokines were tested by real time Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The relative expression levels showed the same trend of expression difference. Significant up-regulation of TMEM173 plays an important role in the cytosolic DNA-sensing pathway and the RIG-I-like receptor signaling pathway in TJM-F92 infected PAM cells. These data provide new insight into PRRSV pathogenicity and immune evasion strategies.