Open AccessChemical composition and anti-Mayaro virus activity of Schinus terebinthifolius fruits
Author(s) -
Tiago Souza Salles,
Marcelo Damião Ferreira de Meneses,
Kristie Aimi Yamamoto,
Thayane Encarnação Sá-Guimarães,
Lúcio Ayres Caldas,
Jessica H. S. Silva,
Polianna da Silva Ferreira,
Aná Claudia F. Amaral,
José Aires Ventura,
Renata Campos Azevedo,
Ricardo Machado Küster,
Márcia Regina Soares,
Davis Fernandes Ferreira
Publication year - 2021
Publication title -
virusdisease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.375
H-Index - 22
eISSN - 2347-3517
pISSN - 2347-3584
DOI - 10.1007/s13337-021-00698-z
Subject(s) - biflavonoids , ethyl acetate , chemistry , composition (language) , traditional medicine , virus , chromatography , biology , virology , stereochemistry , medicine , philosophy , linguistics
Brazilian traditional medicine has explored the antiviral properties of many plant extracts, including those from the Brazilian pepper tree, Schinus terebinthifolius . In the present study, we investigated the chemical composition and anti-mayaro virus (MAYV) activity of S. terebinthifolius fruit. Extensive virucidal activity (more than 95%) was detected for the ethyl acetate extract and the isolated biflavonoids. From the ethyl acetate extract of Schinus terebinthifolius fruits, two bioflavonoids were isolated ((2S, 2″S)-2,3,2″,3″-tetrahydroamentoflavone and agathisflavone), which showed strong virucidal activity against Mayaro virus. Furthermore, several other compounds like terpenes and phenolics were identified by hyphenated techniques (GC-MS, LC-MS and HPLC-UV), as well as by mass spectrometry. Immunofluorescence assay confirmed antiviral activity and transmission electron microscopy revealed damage in viral particles treated with biflavonoids. The data suggest the direct action of the extract and the biflavonoids on the virus particles. The biflavonoids tetrahydroamentoflavone and agathisflavone had strong virucidal activity and reduced MAYV infection.