Open AccessAssociation of cucurbit aphid-borne yellows virus with cucumber plants in India
Author(s) -
Ashwani Kumar,
Ajay Bhattarai,
Aayushi Singh Rathore,
Gograj Singh Jat,
Tusar Kanti Behera,
P. Renukadevi,
V. K. Baranwal,
Rakesh Jain,
Y. B. Basavaraj
Publication year - 2021
Publication title -
virusdisease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.375
H-Index - 22
eISSN - 2347-3517
pISSN - 2347-3584
DOI - 10.1007/s13337-020-00645-4
Subject(s) - bitter gourd , biology , aphid , vigna , cucumis , gourd , cucumber mosaic virus , begomovirus , momordica , plant virus , veterinary medicine , botany , virus , virology , horticulture , traditional medicine , medicine
Symptoms like bright yellowing, puckering of the leaf, vein banding, and vein thickening were observed on different cucurbit hosts at the experimental farm of Indian Agricultural Research Institute, New Delhi during Kharif 2019. Leaf-dip electron microscopy of the symptomatic leaves revealed the association of isometric virus particles measuring ~ 25 nm with bitter gourd and cucumber samples. The RT-PCR assay using polerovirus generic primers covering the partial RdRp, intergenic region, and partial CP region was resulted the amplicons of ~ 1.1 kb. Subsequent cloning, sequencing, and sequence analysis revealed the association of cucurbit aphid-borne yellows virus (CABYV) with bitter gourd (Momordica charantia) and cucumber ( Cucumis sativus ) plants. These results constitute the first report of CABYV infection on cucumber plants from India.