Open AccessGlutinous rice (Oryza sativa L.) protein extract with potent α-amylase inhibitory activity
Author(s) -
Rakrudee Sarnthima,
Saranyu Khammuang,
Anupong Joompang
Publication year - 2020
Publication title -
journal of food science and technology/journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.656
H-Index - 68
eISSN - 0975-8402
pISSN - 0022-1155
DOI - 10.1007/s13197-020-04560-w
Subject(s) - oryza sativa , amylase , rice protein , ic50 , inhibitory postsynaptic potential , chemistry , food science , biochemistry , biology , botany , enzyme , in vitro , gene , endocrinology
This research screened for α-amylase inhibitory activity of twenties-five varieties Thai indigenous rice seeds. Based on specific inhibition, crude protein of var. Gai Ngaw (Gs. No. 13719) was selected for purification. The unbound proteins of the Q-Sepharose column named partially purified rice α-amylase inhibitor (RAI) revealed MW of approximately 14.4 kDa. The RAI was stable at pH 4 to 7 and heat stable up to 80 °C. The RAI had IC 50 of 15.92 ± 1.08 µg/ml. The double reciprocal plot implied a mixed-type inhibitor. The Dixon and Cornish-Bowden plots were used to estimate K i and α K i. This suggested Thai indigenous rice seeds could potentially be developed as a food supplement for blood sugar and weight controls.