Open AccessDiagnosing the drug resistance signature in Plasmodium falciparum: a review from contemporary methods to novel approaches
Author(s) -
Laxman Kumar Murmu,
Arpita Arsmika Sahu,
Tapan Kumar Barik
Publication year - 2021
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01333-2
Subject(s) - plasmodium falciparum , malaria , biology , drug resistance , single nucleotide polymorphism , snp , genome , genetics , population , virology , parasite hosting , computational biology , gene , medicine , immunology , genotype , environmental health , world wide web , computer science
The genome sequence project of the human malaria parasite Plasmodium falciparum reveal variations in the parasite DNA sequence. Most of these variations are single nucleotide polymorphism (SNP). A high frequency of single nucleotide polymorphism (SNP) in the Plasmodium falciparum population is usually a benchmark for anti-malarial resistance which allows parasites to be elusive to the chemotherapeutic agents, vaccine and vector control strategies, resulting in the leading cause of morbidity and mortality globally. The high density of drug resistance signature markers such as pfcrt,pfmdr1, pfdhps, pfdhfr , pfkelch13, pfatpase6 and pfmrp1 in the genome opens up a scope for the study of the genetic basis of this elusive parasite. The precise and prompt diagnosis of resistance strains of parasite plays vital role in malaria elimination program.This review probably shed light on contemporary SNP diagnostic tools used in molecular surveillance of Plasmodium falciparum drug resistance in terms of mechanism, reaction modalities, and development with their virtues and shortcomings.