Open AccessZoonotic assemblages of Giardia duodenalis in captive non-human primates from the largest zoo in Slovakia
Author(s) -
Kristína Mravcová,
Gabriela Štrkolcová,
Rastislav Mucha,
M. Goldová
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01324-3
Subject(s) - biology , lemur catta , giardia , zoology , genotyping , glutamate dehydrogenase , zoonosis , veterinary medicine , parasite hosting , genotype , primate , virology , lemur , microbiology and biotechnology , ecology , genetics , gene , medicine , glutamate receptor , receptor , world wide web , computer science
Only a few studies based on multilocus characterization have been conducted on the molecular epidemiology of Giardia duodenalis in captive nonhuman primates (NHPs). The present article provides the first report on the occurrence of G. duodenalis in the ring-tailed lemur ( Lemur catta ) and barbary macaque ( Macaca sylvanus ) kept in the zoo in Košice, Slovakia. All samples were examined by flotation technique, with total prevalence of 17.4% (4/23). The microscopically positive samples were assayed by nested PCR and consecutively sequenced at β-giardin ( bg ), glutamate dehydrogenase ( gdh ) and triosephosphate isomerase ( tpi ) genes. Positive isolates were recognized as assemblage B and sub-assemblage BIV and subtype WB8 were confirmed. The identification and genotyping of this parasite in Slovakia, may help to better understand the epidemiological situation in Europe about the circulation of G. duodenalis zoonotic assemblages in NHPs.