Open AccessScolicidal effects of Cassia fistula and Urtica dioica extracts on protoscoleces of hydatid cysts
Author(s) -
Amirmehdi Sarvestani,
Ali Akbar Karimian,
Rasool Mohammadi,
Kourosh Cheraghipour,
Masoomeh Zivdri,
Morteza Nourmohammadi,
Mohammad Almasian,
Amirhossein Nafari
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01273-x
Subject(s) - urtica dioica , cassia , traditional medicine , decoction , fistula , medicine , echinococcus granulosus , artemisia , biology , pathology , surgery , traditional chinese medicine , zoology , alternative medicine
Echinococcosis is among the most underestimated parasitic diseases that have universal distribution. The primary treatment is surgery. Hence, the development of new and more effective scolicidal agents with lower side effects is crucial. This study evaluated the therapeutic effects of Urtica dioica and Cassia fistula extracts as a scolicidal herbal drug in vitro. Suspension of protoscoleces was obtained from the infected livers of sheep in Khorramabad, Iran. Hydro-alcoholic solution was extracted from the leaves and stems of Urtica dioica and the fruit of Cassia fistula . Echinococcus granulosus protoscoleces were treated with the essential oils at concentrations of 10, 25, 50, and 100 mg/mL for 10, 20, 30, and 60 min and their viability was evaluated by the eosin staining test. The extract of Urtica dioica at a concentration of 100 mg/mL killed 90.51% of protoscoleces after 60 min. Cassia fistula also killed 67.74% of protoscoleces after 60 min. This study obtained satisfactory results. Urtica dioica and Cassia fistula extracts are promising protoscolicides and can be used in the treatment of hydatid cysts and pre-surgically to prevent secondary infections.