Open AccessMolecular characterization of 5S ribosomal spacer sequences of Dirofilaria repens microfilariae in dogs in Kerala, India
Author(s) -
Deepa Chirayath,
P.C. Alex,
Usha Narayana Pillai
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01257-x
Subject(s) - dirofilaria repens , internal transcribed spacer , ribosomal rna , biology , ribosomal dna , repens , dirofilaria , dirofilariasis , genetics , phylogenetic tree , helminths , zoology , botany , gene , dirofilaria immitis
The present study reports the characteristics of the 5S rRNA spacer sequences of Dirofilaria repens microfilariae isolated from dogs. The 22 nucleotide long spliced leader 1 sequences located in the 5S rRNA spacer region are completely conserved in all nematodes. There is variation in the spliced leader 1 sequences and associated sites in the 5S rRNA spacer region of D. repens . Absence of canonical SL 1 sequences distinguishes D. repens from other filarial species.