Open AccessFrequency of different genotypes of Giardia duodenalis in slaughtered sheep and goat in east of iran
Author(s) -
Ashkan Faridi,
Amir Tavakoli Kareshk,
Saeed Sadooghian,
Nima Firouzeh
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01237-1
Subject(s) - genotype , biology , giardia , nested polymerase chain reaction , veterinary medicine , restriction enzyme , feces , glutamate dehydrogenase , polymerase chain reaction , zoonosis , restriction fragment length polymorphism , dna extraction , digestion (alchemy) , microbiology and biotechnology , gene , virology , genetics , medicine , glutamate receptor , receptor , chemistry , chromatography
Giardia duodenalis is one of the most common and important protozoan parasites of the gastrointestinal tract in humans and animals, especially in developing countries. The purpose of this study was determining prevalence of Giardia genotypes specially zoonosis genotypes in sheep and goat in eastern of iran slaughterers.This cross-sectional study was conducted during April to November 2019. 300 fecal samples were collected from the rectum of sheep and goats. The samples were subjected to DNA extraction after sucrose gradient purification. A fragment of the glutamate dehydrogenase gene ( gdh ) was amplified by semi-nested PCR and genotype diagnosis was performed by digestion of the secondary PCR product with restriction enzymes Rsa I and Nla IV . The prevalence of Giardia was found as (274/300) by the molecular method. Restriction endonuclease digestion of the nested-PCR product showed; among 274 positive isolates, 95 were typed as assemblage E, 15 as assemblage B, 87 assemblage AI, 45 assemblage AII, and 32 assemblege C. In this study, frequency of different assemblages of G. duodenalis was determined in sheep and goats by gdh gene and PCR-RFLP method. Same of other studies, assemblage E was dominant genotype in sheep and goats. Isolation of zoonotic assemblages as AI, AII, and BIII showed that sheep and goats should be considered as a source for human infection.