Open AccessComparison of various methods for DNA extraction from human isolated paraffin-embedded hydatid cyst samples
Author(s) -
Afshin Barazesh,
Moradali Fouladvand,
Mohammad Rayani,
Sepideh Ebrahimi
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01236-2
Subject(s) - dna extraction , hydatid cyst , dna , genomic dna , extraction (chemistry) , chromatography , yield (engineering) , biology , materials science , cyst , pathology , polymerase chain reaction , chemistry , medicine , gene , biochemistry , metallurgy
Successful molecular research with reliable results depends on achieving significant and uniform amounts of genomic DNA from the parasite as the first and most basic step. Therefore, selection of an appropriate method that minimizes damage to the DNA of the parasite, is very important. In this study, we are going to describe a method that can extract DNA from human isolated paraffin-embedded hydatid cysts with a high quality and quantity. Formalin fixed and Paraffin-embedded hydatid cyst samples isolated from human lung and archived in the pathology laboratory were used for this purpose. Several sections of the paraffin blocks were prepared with 5 micron thickness and DNA were extracted by three different methods including; modified boiling, commercial kit and the method described by Larissa A. Pikor et al. The obtained DNA were evaluated by Nanodrop in terms of the yield of DNA and possible contaminations. To compare the quality of DNA prepared, cox 1 region was amplified using specific primers. It was found that the DNA extracted by modified boiling had the lowest rate of contamination and the best electrophoretic band on the gel, compared to other two performed methods. Considering the findings of this study, this simple and high throughput DNA extraction method with high yield and quality can be recommended for extraction of DNA from formalin fixed and paraffin-embedded hydatid cysts.