Open AccessMorphologic and molecular analysis of Dicrocoelium dendriticum species from Iranian native sheep
Author(s) -
Laya Shamsi,
Mousa Tavassoli,
Soraya Naem,
Alireza Mahmoudian,
Elham Ahmadi
Publication year - 2020
Publication title -
journal of parasitic diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.357
H-Index - 21
eISSN - 0975-0703
pISSN - 0971-7196
DOI - 10.1007/s12639-020-01201-z
Subject(s) - genbank , biology , genetic variation , gene , veterinary medicine , zoology , genetics , medicine
Dicrocoeliosis is a worldwide parasitic disease of ruminants which affects the liver. In this current study, the phylogenic pattern of Dicrocoelium species in Iranian native sheep from three different geographical regions was investigated by investigating a 520 bp fragment of mitochondirial NAD1 gene. The analysis of the NAD1 oligo nucleotide sequences from 10 D. dendriticum (GenBank accession numbers: MG889399 to MG889408) revealed few non-significant differences, suggesting limited application for NAD1 gene as a biomarker for study of genetic variation in Dicrocoelium . The morphometrical study also showed a significant relationship for the several morphometric indices among the Dicrocoelium spp. isolates from different regions of Iran.