Open AccessThe effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage
Author(s) -
Pazoki Hassan,
Eimani Hussein,
Farokhi Farah,
Shahverdi AbdolHossein,
Tahaei Leila Sadat
Publication year - 2016
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1007/s12522-015-0220-x
Subject(s) - germinal vesicle , human fertilization , andrology , embryogenesis , embryo , microbiology and biotechnology , reproductive medicine , stage (stratigraphy) , platelet lysate , seminal vesicle , chemistry , platelet , oocyte , biology , medicine , immunology , anatomy , pregnancy , genetics , paleontology , prostate , cancer
Purpose Improving in vitro maturation could increase the rate of pregnancy from oocytes matured in vitro. Consequently, patients will be prevented from using gonadotropin with its related side effects. In this study, the maturation medium was enriched by platelet lysate (PL), then maturation and subsequent developments were monitored. Methods Oocytes at germinal vesicle stage with cumulus cells (cumulus–oocyte complex) and without cumulus cells (denuded oocytes) were obtained from mature female mice. The maturation medium was enriched by 5 and 10 % PL and 5 % PL + 5 % fetal bovine serum (FBS) as experimental groups; the control groups’ media consisted of 5 and 10 % FBS. After 18 h, the matured oocytes were collected and, after fertilization, subsequent development was monitored. Results The rates of maturation, fertilization and 2‐cell embryo development for the denuded oocyte groups in experimental media 5 % PL and 5 % PL + 5 % FBS were significantly higher than those of the control groups ( P < 0.05), while the results for the cumulus–oocyte complex groups were similar between the experimental groups and control groups. Conclusions The results of this study indicated that platelet lysate could improve the maturation rate in the absence of granulosa cells compared to media with FBS. This extract also had positive effects on fertilization and embryo development.