Open AccessCrude bark extract of Dysozylum alliarium induces alteration in histological structures and VEGF‐C expression in uterus during days 4–7 of gestation in albino rat
Author(s) -
Das Moushumi,
Saikia Purba Jyoti,
Sarma Hirendra N.
Publication year - 2013
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1007/s12522-012-0143-8
Subject(s) - uterus , embryo , gestation , andrology , estrous cycle , histology , vascular endothelial growth factor , endocrinology , biology , in vivo , immunohistochemistry , medicine , pregnancy , vegf receptors , genetics , microbiology and biotechnology
Purpose Crude bark extract (CBE) of Dysoxylum alliarium was tested in vivo for its effects on rat uterus during estrus and day 4–7 of gestation. The purpose is to study the effects of CBE on embryo implantation and VEGF‐C expression during peri‐implantation period to find out possible role of VEGF‐C and embryo implantation. Methods Threshold was determined by multiple‐dose administration of CBE. CBE prepared in methanol was orally administered in a dose of 500 mg/kg/day to female rats during day 1–7 of gestation. Routine H&E histology of uteri was studied during estrus phase and days 4–7 of gestation. The vascular endothelial growth factor (VEGF‐C) was studied immunohistochemically and via western blot using VEGF‐C antibody. Results The crude bark extract induced the structural aberration of uterine histology in cyclic stage and during peri‐implantation. Cellular degeneration of embryo and supporting maternal tissue caused loss of embryo minimizing the litter size. Expression of VEGF‐C was drastically reduced in the CBE treated females’ uteri than that of the controls. Conclusions CBE contains potential compounds capable of fertility control as post‐coital contraceptive. Effects of CBE on embryo and foetal‐maternal interface either mediated through reduced VEGF‐C expression or vice versa.